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superscript iii cellsdirect cdna synthesis system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript iii cellsdirect cdna synthesis system
    Superscript Iii Cellsdirect Cdna Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+cellsdirect+cdna+synthesis+system/GLYCOPYRRONIUM+BROMIDE/pmc12702026-52-13-20
    Average 94 stars, based on 1 article reviews
    superscript iii cellsdirect cdna synthesis system - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: ADAMTS7, a target in atherosclerosis, cooperates with its homolog ADAMTS12 to protect against myxomatous valve degeneration
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed into cDNA with SuperScript III Cellsdirect cDNA synthesis system (46-6321, Invitrogen). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for qRT-PCR using an Applied Biosystems 7500 instrument.

    Article Title: Human Embryonic Stem Cells Derived from Embryos at Different Stages of Development Share Similar Transcription Profiles
    Article Snippet: .. RNA was isolated using RNAeasy Plus Minikit (Qiagen Inc., Valencia, CA, USA) and converted to cDNA using SuperScript III CellsDirect cDNA Synthesis System (Invitrogen). .. The polymerase chain reaction (PCR) was performed using Platinum PCR SuperMix (Invitrogen) and primers for transcripts of markers from all three germ layers: T (Brachyury protein), WT1 (Wilms tumor) and ACTA2 (smooth muscle actin, α2) were chosen as markers of mesoderm, GATA4 (GATA binding protein 4), SOX17 (sex determining region Y – box 17) and AFP (α fetoprotein) as markers of endoderm, and TUBB3 (Tubulin, β3), NES (nestin) and PAX6 (paired box 6) as markers of ectoderm.

    Article Title: Restrictive Expression of Acid-Sensing Ion Channel 5 (Asic5) in Unipolar Brush Cells of the Vestibulocerebellum
    Article Snippet: .. RNA from individual cells was isolated and reverse transcribed using the SuperScript III CellsDirect cDNA Synthesis System (Life Technologies). .. PCR was then performed as above with the iProof High Fidelity PCR Kit (Bio-Rad Laboratories).

    Article Title: Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development.
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen, Thousand Oaks, CA). .. Power SYBR Green Mastermix (4367659, Applied Biosystems, Foster City, CA) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Article Title: ADAMTS7, a target in atherosclerosis, cooperates with its homolog ADAMTS12 to protect against myxomatous valve degeneration
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed into cDNA with SuperScript III Cellsdirect cDNA synthesis system (46-6321, Invitrogen). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for qRT-PCR using an Applied Biosystems 7500 instrument.

    Article Title: Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen, Thousand Oaks, CA). .. Power SYBR Green Mastermix (4367659, Applied Biosystems, Foster City, CA) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Reverse Transcription:

    Article Title: ADAMTS7, a target in atherosclerosis, cooperates with its homolog ADAMTS12 to protect against myxomatous valve degeneration
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed into cDNA with SuperScript III Cellsdirect cDNA synthesis system (46-6321, Invitrogen). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for qRT-PCR using an Applied Biosystems 7500 instrument.

    Article Title: Generation of a conditional Adamts6 mouse allele reveals roles in lung maturation in addition to cardiac and musculoskeletal development
    Article Snippet: .. Following tissue homogenization in TRIzol (15596018, Invitrogen) total RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen). cDNA was generated from 1 μg RNA using a High-Capacity cDNA reverse transcription kit following the manufacturer’s instructions (4368814, Applied Biosystems). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Article Title: Restrictive Expression of Acid-Sensing Ion Channel 5 (Asic5) in Unipolar Brush Cells of the Vestibulocerebellum
    Article Snippet: .. RNA from individual cells was isolated and reverse transcribed using the SuperScript III CellsDirect cDNA Synthesis System (Life Technologies). .. PCR was then performed as above with the iProof High Fidelity PCR Kit (Bio-Rad Laboratories).

    Article Title: Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development.
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen, Thousand Oaks, CA). .. Power SYBR Green Mastermix (4367659, Applied Biosystems, Foster City, CA) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Article Title: ADAMTS7, a target in atherosclerosis, cooperates with its homolog ADAMTS12 to protect against myxomatous valve degeneration
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed into cDNA with SuperScript III Cellsdirect cDNA synthesis system (46-6321, Invitrogen). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for qRT-PCR using an Applied Biosystems 7500 instrument.

    Article Title: Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen, Thousand Oaks, CA). .. Power SYBR Green Mastermix (4367659, Applied Biosystems, Foster City, CA) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    cDNA Synthesis:

    Article Title: ADAMTS7, a target in atherosclerosis, cooperates with its homolog ADAMTS12 to protect against myxomatous valve degeneration
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed into cDNA with SuperScript III Cellsdirect cDNA synthesis system (46-6321, Invitrogen). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for qRT-PCR using an Applied Biosystems 7500 instrument.

    Article Title: Human Embryonic Stem Cells Derived from Embryos at Different Stages of Development Share Similar Transcription Profiles
    Article Snippet: .. RNA was isolated using RNAeasy Plus Minikit (Qiagen Inc., Valencia, CA, USA) and converted to cDNA using SuperScript III CellsDirect cDNA Synthesis System (Invitrogen). .. The polymerase chain reaction (PCR) was performed using Platinum PCR SuperMix (Invitrogen) and primers for transcripts of markers from all three germ layers: T (Brachyury protein), WT1 (Wilms tumor) and ACTA2 (smooth muscle actin, α2) were chosen as markers of mesoderm, GATA4 (GATA binding protein 4), SOX17 (sex determining region Y – box 17) and AFP (α fetoprotein) as markers of endoderm, and TUBB3 (Tubulin, β3), NES (nestin) and PAX6 (paired box 6) as markers of ectoderm.

    Article Title: Generation of a conditional Adamts6 mouse allele reveals roles in lung maturation in addition to cardiac and musculoskeletal development
    Article Snippet: .. Following tissue homogenization in TRIzol (15596018, Invitrogen) total RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen). cDNA was generated from 1 μg RNA using a High-Capacity cDNA reverse transcription kit following the manufacturer’s instructions (4368814, Applied Biosystems). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Article Title: Restrictive Expression of Acid-Sensing Ion Channel 5 (Asic5) in Unipolar Brush Cells of the Vestibulocerebellum
    Article Snippet: .. RNA from individual cells was isolated and reverse transcribed using the SuperScript III CellsDirect cDNA Synthesis System (Life Technologies). .. PCR was then performed as above with the iProof High Fidelity PCR Kit (Bio-Rad Laboratories).

    Article Title: Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development.
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen, Thousand Oaks, CA). .. Power SYBR Green Mastermix (4367659, Applied Biosystems, Foster City, CA) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Article Title: ADAMTS7, a target in atherosclerosis, cooperates with its homolog ADAMTS12 to protect against myxomatous valve degeneration
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed into cDNA with SuperScript III Cellsdirect cDNA synthesis system (46-6321, Invitrogen). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for qRT-PCR using an Applied Biosystems 7500 instrument.

    Article Title: Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development
    Article Snippet: .. Briefly, total RNA was isolated using TRIzol (15596018, Invitrogen), and 1 μg of RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen, Thousand Oaks, CA). .. Power SYBR Green Mastermix (4367659, Applied Biosystems, Foster City, CA) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Tissue Homogenization:

    Article Title: Generation of a conditional Adamts6 mouse allele reveals roles in lung maturation in addition to cardiac and musculoskeletal development
    Article Snippet: .. Following tissue homogenization in TRIzol (15596018, Invitrogen) total RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen). cDNA was generated from 1 μg RNA using a High-Capacity cDNA reverse transcription kit following the manufacturer’s instructions (4368814, Applied Biosystems). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    Generated:

    Article Title: Generation of a conditional Adamts6 mouse allele reveals roles in lung maturation in addition to cardiac and musculoskeletal development
    Article Snippet: .. Following tissue homogenization in TRIzol (15596018, Invitrogen) total RNA was reverse transcribed with SuperScript III CellsDirect cDNA synthesis system (46–6321, Invitrogen). cDNA was generated from 1 μg RNA using a High-Capacity cDNA reverse transcription kit following the manufacturer’s instructions (4368814, Applied Biosystems). .. Power SYBR Green Mastermix (4367659, Applied Biosystems) was used for RT-qPCR using an Applied Biosystems 7500 instrument.

    other:

    Article Title: Expression Analysis of the NLRP Gene Family Suggests a Role in Human Preimplantation Development
    Article Snippet: Oocyte and embryo cDNA was synthesized using SuperScriptTM III CellsDirect cDNA Synthesis system (Invitrogen, Stockholm, Sweden).



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    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control